![[cover image] principles of two photon fluorescence microscopy (image credit: Jian Zhong)](https://jianzhongdev.github.io/VisionTechInsights/images/basic_math_for_two_photon_fluorescence_microscopy/2PFM_Principles.png)
Basic Math for Two Photon Fluorescence Microscopy
It’s really helpful to do some basic math to understand how well an imaging system works when you’re designing or improving it. Most of the time, a very basic model of the system can provide a ton of useful information. Therefore, I would like to share an example of how we can crunch some numbers to understand the signal and noise in a two-photon fluorescence microscope (2PFM). In the end, I will also provide a practical demo of how this math can help us make the system work better....